rabbit anti ip 3 r2  (Alomone Labs)

Journal: International Journal of Molecular Sciences

Article Title: Inositol (1,4,5)-Trisphosphate Receptors in Invasive Breast Cancer: A New Prognostic Tool?

doi: 10.3390/ijms23062962

Figure Lengend Snippet: IP 3 R expression in BC tissue and non-tumor tissue. The relative expression levels of IP 3 R1 and IP 3 R3 are significantly higher in BC tissue than in non-tumor tissue; this difference was not observed for IP 3 R2 ( A ). The same results were obtained when considering the IH expression score ( B ). ( A ) IP 3 R relative expression in IBC-NST, in a Western blot (T: tumor tissue; NT: non-tumor tissue). ( B ) The IP 3 R IH expression score in IBC-NST (T: tumor tissue; NT: non-tumor tissue). ( C ) A representative IH image (magnification: 200 X; insert: 800 X). * p < 0.05; *** p < 0.001.

Article Snippet: Next, the membranes were incubated overnight at 4 °C with mouse monoclonal anti-IP 3 R1 (1/500, Neuromab, CA, USA), rabbit monoclonal anti-IP 3 R2 (1/250, Santa Cruz, CA, USA), mouse monoclonal anti-IP 3 R3 (1/500, BD Biosciences, Switzerland), or goat polyclonal anti-actin (1/2500, Santa Cruz, CA, USA) primary antibodies diluted in 1% BSA in PBS-T. Actin primary antibody was used for loading control experiments.

Techniques: Expressing, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Inositol (1,4,5)-Trisphosphate Receptors in Invasive Breast Cancer: A New Prognostic Tool?

doi: 10.3390/ijms23062962

Figure Lengend Snippet: The IP 3 R IH expression score, as a function of tumor size ( A – C ). The IP 3 R IH expression scores did not differ significantly in T1 BC ( A ). The IP 3 R2 and IP 3 R3 IH expression scores were significantly higher than IP 3 R1 score in T2 and T3 BCs ( B , C ). *** p < 0.001.

Article Snippet: Next, the membranes were incubated overnight at 4 °C with mouse monoclonal anti-IP 3 R1 (1/500, Neuromab, CA, USA), rabbit monoclonal anti-IP 3 R2 (1/250, Santa Cruz, CA, USA), mouse monoclonal anti-IP 3 R3 (1/500, BD Biosciences, Switzerland), or goat polyclonal anti-actin (1/2500, Santa Cruz, CA, USA) primary antibodies diluted in 1% BSA in PBS-T. Actin primary antibody was used for loading control experiments.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Inositol (1,4,5)-Trisphosphate Receptors in Invasive Breast Cancer: A New Prognostic Tool?

doi: 10.3390/ijms23062962

Figure Lengend Snippet: The IP 3 R IH expression scores as a function of lymph node status ( A , B ). *** p < 0.001.

Article Snippet: Next, the membranes were incubated overnight at 4 °C with mouse monoclonal anti-IP 3 R1 (1/500, Neuromab, CA, USA), rabbit monoclonal anti-IP 3 R2 (1/250, Santa Cruz, CA, USA), mouse monoclonal anti-IP 3 R3 (1/500, BD Biosciences, Switzerland), or goat polyclonal anti-actin (1/2500, Santa Cruz, CA, USA) primary antibodies diluted in 1% BSA in PBS-T. Actin primary antibody was used for loading control experiments.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Inositol (1,4,5)-Trisphosphate Receptors in Invasive Breast Cancer: A New Prognostic Tool?

doi: 10.3390/ijms23062962

Figure Lengend Snippet: IP 3 R expression score as a function of the BC molecular subtype. ( A ) Luminal A, ( B ) luminal B, ( C ) triple negative, ( D ) luminal B HER2-, and ( E ) luminal B HER2+ BC. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: Next, the membranes were incubated overnight at 4 °C with mouse monoclonal anti-IP 3 R1 (1/500, Neuromab, CA, USA), rabbit monoclonal anti-IP 3 R2 (1/250, Santa Cruz, CA, USA), mouse monoclonal anti-IP 3 R3 (1/500, BD Biosciences, Switzerland), or goat polyclonal anti-actin (1/2500, Santa Cruz, CA, USA) primary antibodies diluted in 1% BSA in PBS-T. Actin primary antibody was used for loading control experiments.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Inositol (1,4,5)-Trisphosphate Receptors in Invasive Breast Cancer: A New Prognostic Tool?

doi: 10.3390/ijms23062962

Figure Lengend Snippet: IP 3 R expression in non-tumor tissue. ( A ) Western blot analysis of the relative expression of IP 3 R1/2/3. ( B ) The IH expression score for IP 3 R1/2/3. ( C ) Representative IH images. *** p < 0.001.

Article Snippet: Next, the membranes were incubated overnight at 4 °C with mouse monoclonal anti-IP 3 R1 (1/500, Neuromab, CA, USA), rabbit monoclonal anti-IP 3 R2 (1/250, Santa Cruz, CA, USA), mouse monoclonal anti-IP 3 R3 (1/500, BD Biosciences, Switzerland), or goat polyclonal anti-actin (1/2500, Santa Cruz, CA, USA) primary antibodies diluted in 1% BSA in PBS-T. Actin primary antibody was used for loading control experiments.

Techniques: Expressing, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Inositol (1,4,5)-Trisphosphate Receptors in Invasive Breast Cancer: A New Prognostic Tool?

doi: 10.3390/ijms23062962

Figure Lengend Snippet: Analysis of the association between IP 3 R expression and overall survival, after application of the SurvExpress tool to the “ BRCA-TCGA Breast invasive carcinoma — July 2016 ” database (comprising 962 BC samples). ( A , B ) IP 3 R1 expression and IP 3 R3 expression were significantly associated with poor overall survival ( p = 0.0009 and p = 0.003). ( C ) IP 3 R2 expression was not significantly associated with poor overall survival ( p = 0.286). ( D ) The HR for each IP 3 R appears in an analysis of the “ Breast invasive carcinoma — July 2016” database. ** p < 0.01; *** p < 0.001.

Article Snippet: Next, the membranes were incubated overnight at 4 °C with mouse monoclonal anti-IP 3 R1 (1/500, Neuromab, CA, USA), rabbit monoclonal anti-IP 3 R2 (1/250, Santa Cruz, CA, USA), mouse monoclonal anti-IP 3 R3 (1/500, BD Biosciences, Switzerland), or goat polyclonal anti-actin (1/2500, Santa Cruz, CA, USA) primary antibodies diluted in 1% BSA in PBS-T. Actin primary antibody was used for loading control experiments.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Inositol (1,4,5)-Trisphosphate Receptors in Invasive Breast Cancer: A New Prognostic Tool?

doi: 10.3390/ijms23062962

Figure Lengend Snippet: Statistically significant correlations between IP 3 R subtype expression and survival. IP 3 R3 and IP 3 R1 expression levels were significantly associated with worse overall survival in three datasets (comprising 1358 patients (A) and 1700 patients (B), respectively), and IP 3 R2 was significantly associated with worse overall survival in two datasets (comprising 434 patients) (C). Expression of the three IP 3 R subtypes was significantly associated with worse recurrence-free survival in two datasets (comprising 3449 patients) (D). HR: hazard ratio. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: Next, the membranes were incubated overnight at 4 °C with mouse monoclonal anti-IP 3 R1 (1/500, Neuromab, CA, USA), rabbit monoclonal anti-IP 3 R2 (1/250, Santa Cruz, CA, USA), mouse monoclonal anti-IP 3 R3 (1/500, BD Biosciences, Switzerland), or goat polyclonal anti-actin (1/2500, Santa Cruz, CA, USA) primary antibodies diluted in 1% BSA in PBS-T. Actin primary antibody was used for loading control experiments.

Techniques: Expressing